Mass Spectrometric Detection of Enzymatic Bioassays
نویسندگان
چکیده
منابع مشابه
Mass Spectrometric Detection of Bacterial Protein Toxins and Their Enzymatic Activity
Mass spectrometry has recently become a powerful technique for bacterial identification. Mass spectrometry approaches generally rely upon introduction of the bacteria into a matrix-assisted laser-desorption time-of-flight (MALDI-TOF) mass spectrometer with mass spectrometric recognition of proteins specific to that organism that form a reliable fingerprint. With some bacteria, such as Bacillus ...
متن کاملMass spectrometric method incorporating enzymatic amplification for attomole-level analysis of target metabolites in biological samples.
We report a sensitive method for selective detection of target metabolites from the central metabolic pathway by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), in which the MS signal is enhanced by up to three orders of magnitude in the course of in situ enzymatic amplification.
متن کاملIn-house validation of a method for determination of silver nanoparticles in chicken meat based on asymmetric flow field-flow fractionation and inductively coupled plasma mass spectrometric detection.
Nanomaterials are increasingly used in food production and packaging, and validated methods for detection of nanoparticles (NPs) in foodstuffs need to be developed both for regulatory purposes and product development. Asymmetric flow field-flow fractionation with inductively coupled plasma mass spectrometric detection (AF(4)-ICP-MS) was applied for quantitative analysis of silver nanoparticles ...
متن کاملIsotope dilution--mass spectrometric quantification of specific proteins: model application with apolipoprotein A-I.
An enzymatic hydrolysis isotope dilution-mass spectrometric method was developed for reference quantification of specific proteins. The analytical procedure involved measuring a reproducibly hydrolyzed peptide (serving as the primary standard) unique to a specific protein. This new mass spectrometric method was evaluated by assessing the concentration of apolipoprotein (apo) A-I in the European...
متن کاملFirst Report on Screening of the Profiles of the Essential Oils and Volatiles from the Aerial Parts of Marrubium persicum Using Classical and Advanced Methods Prior to Gas Chromatographic Mass Spectrometric Determination
The Lamiaceae family consists of a broad spectrum of medicinal plants involving Marrubium L. genus. Regarding the diverse pharmaceutical uses of the plants belonging to this genus, they can be considered as proper alternatives for chemical drugs having harmful effects. The present work aims to identify and characterize chemical compositions of the essential oils and volatiles from the aerial pa...
متن کامل